use of chromatographic techniques in the identification of bacteroides species

by M. A. Samara

Written in English
Published: Downloads: 218
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Edition Notes

Thesis(Ph.D.) - Loughborough University of Technology 1983.

Statementby M.A. Samara.
ID Numbers
Open LibraryOL19574046M

The information so generated has a potential application in the identification of an authentic drug, in excluding the adulterants and in maintaining the quality and consistency of the drug. 2. TLC was the common method of choice for herbal analysis before instrumental chromatography methods like GC and HPLC were established. chromatography or a variation thereon. Chromatography is used in many different ways. Some people use chromatography to find out what is in a solid or a liquid. It is also used to determine what unknown substances are. The Police, F.B.I., and other detectives use chromatography when trying to . Background. Thin layer chromatography (TLC) is used routinely in the laboratory to both monitor reactions and analyse the purity of samples. TLC is a type of adsorption chromatography, and the most common substrates used for the stationary phases in the lab, are silica (SiO 2) and alumina (Al 2 O 3).It is recommended that you read the page on adsorption chromatography before doing this experiment. Chromatography is the collective term for a set of laboratory techniques for the separation of purpose of this book is not only to present the latest state and development tendencies of chromatography, but to bring the reader useful information on separation sciences to enable him to use chromatography on his research field.

A two-pronged approach to waste identification through chemical analysis was developed using: (1) select fecal sterol analysis and analysis of concentration ratios and (2) select pharmaceutical and personal care/use product analysis. Both techniques utilize solid phase extraction (SPE) and high. Here we have assessed whether strains within a given Bacteroides species display similar fructan utilization profiles. We carried out an in vitro growth analysis of B. thetaiotaomicron strains present in our culture collection, in the presence of Bacteroides minimal medium (MM) containing either levan (% [wt/vol]) or inulin (% [wt/vol. From the enormous variety of methods of separation and isolation useful for natural products, adsorption or partition chromatography represents one of the most useful techniques of general application. Thin layer chromatographic (TLC) is the simplest technique used to separate and identify . Gas chromatography–mass spectrometry (GC-MS) is an analytical method that combines the features of gas-chromatography and mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, fire investigation, environmental analysis, explosives investigation, and identification of unknown samples, including that of material samples obtained.

  The role of dietary fiber in supporting healthy gut microbiota and overall well-being of the host has been revealed in several studies. Here, we show the effect of a bacterial polyfructan levan on the growth dynamics and metabolism of fecal microbiota in vitro by using isothermal microcalorimetry. Eleven fecal samples from healthy donors were incubated in phosphate-buffered defined medium with.

use of chromatographic techniques in the identification of bacteroides species by M. A. Samara Download PDF EPUB FB2

Also pyrolysis gas chromatography of whole cells was used, with special reference to the ability of this technique in identifying bacteroides organisms involved in an infection process. Biochemical techniques showed the use of API 20A, the miniaturised system of identification, to be superior to the conventional methods for its more consistant Author: Mahira A.

Samara. Abstract. A Doctoral Thesis. Submitted in partial fulfilment of the requirements for the award of Doctor of Philosophy of Loughborough fication of various Bacteroides species studied in this thesis employed a range of morphological\ud biochemical and some chemotaxonomic techniques such as\ud short chain fatty acid analysis by gas chromatography.\ud Also pyrolysis gas Author: Mahira A.

Samara. Abstract. From 56 strains of strictly anaerobic gram-negative rods isolated from stool and purulent lesions the fermentation products in the presence and absence of hemin were determined by quantitative gas-solid chromatography, using a simple and more rapid chromatographic by: Abstract.

A major application of chromatography in microbiology has been the study of the chemical composition of microorganisms. Data from these studies use of chromatographic techniques in the identification of bacteroides species book demonstrated the value of chemical information for differentiation of closely related groups or species of by: 3.

A.A. Salyers, N.B. Shoemaker, in Encyclopedia of Microbiology (Third Edition), The Definition of Bacteroides as a Genus and a Phylogenetic Group. Bacteroides species were the first Gram-negative anaerobes to be viewed as potentially serious pathogens.

Accori-dngly, more is known about their traits and ability to cause disease than about the other Gram-negative anaerobes. Separation of species by thin-layer chromatography Analysis of the composition of some common medicines. Method dichloromethane ibuprofen tablet paracetamol tablet caffeine tablet aspirin tablet Anadin Extra tablet (or equivalent containing aspirin, paracetamol and caffeine) pestle and mortar TLC plate capillary tubes developing chamber (or suitable container with lid) access to UV lamp.

10 and liquid chromatography is mass spectrometry (MS) which transforms each 11 analyte from a chemically neutral species into a positive cation, usually breaking 12 various bonds in the process. Detecting the mass of the individual pieces 13 (referred to as fragments) allows for conclusive identification of the chemical.

SUMMARY Summary: Bacteroides species are significant clinical pathogens and are found in most anaerobic infections, with an associated mortality of more than 19%. The bacteria maintain a complex and generally beneficial relationship with the host when retained in the gut, but when they escape this environment they can cause significant pathology, including bacteremia and abscess formation in.

As in most other forms of chromatography, paper chromatography uses R n values to help identify compounds. R f values are calculated by dividing the distance the pigment travels up the paper by the distance the solvent travels (the solvent front).

Because R f values are standard for a given compound, known R n values can be used to aid in the identification of an unknown substance in an. Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis.

Bacterial Identification methods. Bacteria as mentioned before or so tiny that one cannot watch without a microscope. So either we identify them using a microscope or by enhancing their population to a huge bulk or by biochemical methods.

Hence in the identification of bacteria, we use microscopical, medical, biochemical, & serological methods. Bacteroides fragilis and related species are important human pathogens involved in mixed infections of different origins. The B. fragilis group isolates are phenotypically very similar, grow more slowly than aerobic bacteria and, accordingly, are frequently misidentifed with classical or automated phenotypical identification methods.

Recent taxonomic changes and new species accepted as members. Anaerobic bacteria can cause a wide variety of infections, and some of these infections can be serious. Conventional identification methods based on biochemical tests are often lengthy and can produce inconclusive results.

An oligonucleotide array based on the 16SS rRNA intergenic spacer (ITS) sequences was developed to identify 28 species of anaerobic bacteria and Veillonella.

Ion exchange mechanism. Ion-exchange chromatography which is designed specifically for the separation of differently charged or ionizable compounds comprises from mobile and stationary phases similar to other forms of column based liquid chromatography techniques [].Mobil phases consist an aqueous buffer system into which the mixture to be resolved.

Bacteroides is a genus of Gram-negative, obligate anaerobic bacteria. Bacteroides species are non endospore-forming bacilli, and may be either motile or nonmotile, depending on the species. The DNA base composition is 40–48% l in bacterial organisms, Bacteroides membranes contain also contain meso-diaminopimelic acid in their peptidoglycan layer.

A simple method for the separation and identification of Bacteroides fragilis from other Bacteroides species through the use of oxgall-impregnated filter paper disks is described. In some cases, technicians will use reverse-phase where the stationary phase is non-polar while the mobile phase is polar.

Although there are different types of chromatography that vary depending on the type of stationary and mobile phase used, the basic principle is the same. Chromatography was first devised in Russia by the Italian-born scientist Mikhail Tsvet in He developed the technique, he coined chromatography, in the first decade of the 20th century, primarily for the separation of plant pigments such as chlorophyll, carotenes, and these components separate in bands of different colors (green, orange, and yellow, respectively) they.

based electrophoretic techniques. This book will provide a basic introduction to different types of liquid and gas chromatography. The relationship between each type of chromatography is illustrated in Figure Figure Categories of Chromatography and Their Relationship to Each Other.

Chromatography The Most Versatile Method of Chemical Analysis. Chromatography is the collective term for a set of laboratory techniques for the separation of purpose of this book is not only to present the latest state and development tendencies of chromatography, but to bring the reader useful information on separation sciences to enable him to use chromatography on his research.

Obligately anaerobic, Gram-negative rods of clinical relevance belong mainly to the phyla Bacteroidetes and Fusobacteria. Anaerobes are detected typically in polymicrobial infections associated with mucosal surfaces close to the site where they reside; e.g., members of the Bacteroides fragilis group are often involved in intra-abdominal infections, with considerable morbidity and mortality.

Chromatography is one of several separation techniques defined as differential migration from a narrow initial zone. Electrophoresis is another member of this group. In this case, the driving force is an electric field, which exerts different forces on solutes of different ionic charge.

The resistive force is the viscosity of the nonflowing. The Vitek MS database is more specific for the identification of Streptococcus viridans. For the anaerobes, the Biotyper database often identified Fusobacterium isolates to only the genus level, which is of low clinical significance, whereas 20% of the Bacteroides species were not identified or were misidentified by the Vitek MS database.

The chromatographic techniques help in the use of volatile compounds analysis. The isotope analysis helps in distinguishing between chicken meat and vegetable oils.

Ambient mass spectrometry is used for studying mycotoxines and alkaloids in foodstuffs and their management, food and feed authentication in olive and other plant oils, and wine. API 20A correctly identified % of strains to species and % to genus only, with % as part of a spectrum of identifications and % incorrect.

Minitek, according to the current code book, yielded % correct identifications to species, % spectrum identifications, and % incorrect. The molecular species subjected to separation exist in a sample that is made of analytes and matrix. The analytes are the molecular species of interest, and the matrix is the rest of the components in the sample.

For chromatographic separation, the sample is introduced in a flowing mobile phase that passes a stationary phase. Chromatography is used in industrial processes to purify chemicals, test for trace amounts of substances, separate chiral compounds and test products for quality tography is the physical process by which complex mixtures are separate.

Purchase Lipid Analysis - 4th Edition. Print Book & E-Book. ISBNBiological chromatographic fingerprinting is a relatively new concept in the quality control of herbal samples. Originally it has been developed with the application of HPLC, and recently herbal samples' biological profiles have been obtained by means of thin-layer chromatography (TLC).

This paper summarizes the application of liquid chromatographic techniques for the purpose of biological. High-performance liquid chromatography (HPLC), formerly referred to as high-pressure liquid chromatography, is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture.

It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Typically, the chromatography is performed using buffers at pH's between 7 and 10 and running a gradient from a solution containing just this buffer to a solution containing this buffer with 1M NaCl.

The salt (in solution) competes for binding to the stationary phase, thus releasing the .Basic Bacterial Identification by Microscopy, Gram-positive or Gram-negative, coccus or bacillus, this page helps with bacterial identification, Bacterial Causes of Infection, Gram film appearance and microscopy, A microbiology and infection teaching reso.liquid chromatography chapter 14 advanced spectroscopic detectors for identification and quantification mass spectrometry Posted By William Shakespeare Media TEXT ID cb4d7e Online PDF Ebook Epub Library the rector magnificus prof dr liquid chromatography chapter 14 advanced spectroscopic detectors for identification and quantification mass spectrometry kindle .